Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155849_lhx0 TCTCGGGGCTAGCTGGTCATGCCATCCTCACG(+AGGACA)GATCCCCAAGCCATCTCCAAACACCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613155848_86b7 CCCCTTTCATGGTCCGTTTTCTTTTCAAACTG(+AGGACA)GATCCAAAAAGAGACGAGGGTTTTCTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613155847_82u3 CCTAGAGGAAAGAAAACCCTCGTCTCTTTTTG(+AGGACA)GATCCAGTTTGAAAAGAAAACGGACCATGAA NGG 10 8 17 8 24 0 100 5 10
    240613155846_wrr6 CTGGTAAAACTACTGCAGTGGGGCTTTGACAG(+AGGACA)GATCCAAGTTATCACCAGAAAACAAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240613155844_wbpk CACTTTTTGTTTTGTTTTCTGGTGATAACTTG(+AGGACA)GATCCTGTCAAAGCCCCACTGCAGTAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613155843_h7k2 CCAAATACAGAAAAGAGGAGGCCTGAAAGAGG(+AGGACA)GTACCAGGGGACAGGGAGCCATGGGGGCCTA NGG 10 8 17 8 24 0 100 5 10
    240613155840_d1mj AACCCTAGGCCCCCATGGCTCCCTGTCCCCTG(+AGGACA)GTACCCTCTTTCAGGCCTCCTCTTTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613155838_qqmu ATTCCCAGTCAGCTTCAGGGGAATGAGAAAGG(+AGGACA)GATCCTACTAATGTGGAAAGAAGCAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240613155837_t3hx GGAGGGATGCTTGCTTCTTTCCACATTAGTAG(+AGGACA)GATCCCTTTCTCATTCCCCTGAAGCTGACTG NGG 10 8 17 8 24 0 100 5 10
    240613155835_6uo8 CCTTGCTGTTAATTTTGCATATGCTAAAGCGG(+AGGACA)GATCCCCTGGAGGCGGCATTTTCCCTTAGCT NGG 10 8 17 8 24 0 100 5 10