Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220502_3gxb ACTTGAATCTCACGTGGCAGGAGGGGCTATGG(+ACTCAC)GATCCGCTCGTCTTCCTCACTGCCGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240604220501_cvf3 GGGGAGCTGTGCGGCAGTGAGGAAGACGAGCG(+ACTCAC)GATCCCATAGCCCCTCCTGCCACGTGAGATT NGG 10 8 17 8 24 0 100 5 10
    240604220459_albu AATCAACCAATCATATGAAAAAGAACTTTGTG(+ACTCAC)GTACCTAGAGGGGCAGAGCCTGCAAGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240604220458_jihc CAGGGCCCACCTTGCAGGCTCTGCCCCTCTAG(+ACTCAC)GTACCACAAAGTTCTTTTTCATATGATTGGT NGG 10 8 17 8 24 0 100 5 10
    240604220456_zd1l CCCCTGGGAAAGAATTTTTTAAAAGGGCTAGG(+ACTCAC)GTACCATTCATCCAATATTTATAGAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240604220455_hnve TTCTGCCATTTTCTATAAATATTGGATGAATG(+ACTCAC)GTACCCTAGCCCTTTTAAAAAATTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604220453_s4b8 AATACACATGTGCCAAAACTTGTCTGTAGAGG(+ACTCAC)GATCCGACTCATGGGCCGTGTGCGTGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240604220452_lior CTGCCTACTGCACGCACACGGCCCATGAGTCG(+ACTCAC)GATCCCTCTACAGACAAGTTTTGGCACATGT NGG 10 8 17 8 24 0 100 5 10
    240604220450_uhuh AATACACATGTGCCAAAACTTGTCTGTAGAGG(+ACTCAC)GATCCGACTCATGGGCCGTGTGCGTGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240604220449_y40z CTGCCTACTGCACGCACACGGCCCATGAGTCG(+ACTCAC)GATCCCTCTACAGACAAGTTTTGGCACATGT NGG 10 8 17 8 24 0 100 5 10