Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220429_4ltb TGTTTCTGGGGCCTCCTTCACGGATTCATATG(+ACTCAC)GTACCTTCCTTCTAAACTTGAGGAAGGCATT NGG 10 8 17 8 24 0 100 5 10
    240604220427_916v AGACAAATGCCTTCCTCAAGTTTAGAAGGAAG(+ACTCAC)GTACCATATGAATCCGTGAAGGAGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604220425_quyy ACTTCTCCCTGTCAGCCCCAGCTGGCCCATGG(+ACTCAC)GATCCTGGGACAGCCGACTCATGACCCACCT NGG 10 8 17 8 24 0 100 5 10
    240604220423_egec TGTCCAGGTGGGTCATGAGTCGGCTGTCCCAG(+ACTCAC)GATCCCATGGGCCAGCTGGGGCTGACAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604220421_qu23 AGGGCAGCATCTCCTGGGAAAACGAACTAGAG(+ACTCAC)GATCCAGAGCCTTGTGGCCCTTTCACGGTCA NGG 10 8 17 8 24 0 100 5 10
    240604220420_0951 TTCTCTGACCGTGAAAGGGCCACAAGGCTCTG(+ACTCAC)GATCCTCTAGTTCGTTTTCCCAGGAGATGCT NGG 10 8 17 8 24 0 100 5 10
    240604220419_g3t0 GGCTCCCCTGCCTCAGTCTGCAAGGCTACTTG(+ACTCAC)GTACCTCCTCACGCTCCGAGAAGTCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240604220417_b53l GGTAAGTGTGAGACTTCTCGGAGCGTGAGGAG(+ACTCAC)GTACCAAGTAGCCTTGCAGACTGAGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240604220415_le57 GGAGGGGCACCCCACTCCTCTATGGCAACTGG(+ACTCAC)GTACCAAAGTCCTACAGCAGAGGCTACAGCA NGG 10 8 17 8 24 0 100 5 10
    240604220414_49fy ACCCATGCTGTAGCCTCTGCTGTAGGACTTTG(+ACTCAC)GTACCCAGTTGCCATAGAGGAGTGGGGTGCC NGG 10 8 17 8 24 0 100 5 10