Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220356_ze08 CTTGGAGTCCCTAGATTAATGCTGGAGATGGG(+ACTCAC)GATCCCAAGGTCTCCCAATGCCACTGGAGTC NGG 10 8 17 8 24 0 100 5 10
    240604220354_y8fg TCTAGGACTCCAGTGGCATTGGGAGACCTTGG(+ACTCAC)GATCCCCATCTCCAGCATTAATCTAGGGACT NGG 10 8 17 8 24 0 100 5 10
    240604220351_9swm TGGGCTCTATGAATGATGCAACACATTAGATG(+ACTCAC)GTACCTCCAAGGCTGGCTTCTACCATGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604220350_dh7m AGGGTAACTCATGGTAGAAGCCAGCCTTGGAG(+ACTCAC)GTACCATCTAATGTGTTGCATCATTCATAGA NGG 10 8 17 8 24 0 100 5 10
    240604220348_tgop GAGGCTCCAGCCAAGATTGACAGATCCAGGTG(+ACTCAC)GATCCTGATCCATTATTTAGCTATCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604220347_qbe1 TCACTGGAAGAGATAGCTAAATAATGGATCAG(+ACTCAC)GATCCACCTGGATCTGTCAATCTTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604220345_50zh AAAGGGGCAGTGATGCTGAGCTGATCTAGGAG(+ACTCAC)GATCCCTGGACCGTGCTCCATAGCATCAAAC NGG 10 8 17 8 24 0 100 5 10
    240604220343_j9ph ATCTCGTTTGATGCTATGGAGCACGGTCCAGG(+ACTCAC)GATCCTCCTAGATCAGCTCAGCATCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240604220341_5in5 TCTTGATGATAATGGAGAGAGGACCCTGAAAG(+ACTCAC)GTACCTCCAGTTAGCTTAAGCCAGAGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240604220340_k29k AAAGAGGCGACTCTGGCTTAAGCTAACTGGAG(+ACTCAC)GTACCTTTCAGGGTCCTCTCTCCATTATCAT NGG 10 8 17 8 24 0 100 5 10