Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220140_dmbf AAGTCCTCACAGAGGACACAGCTCAGGATCGG(+ACTCAC)GATCCCACACCTGTAAAAAGTAAAGAACAAG NGG 10 8 17 8 24 0 100 5 10
    240604220139_33yh AACCACTTGTTCTTTACTTTTTACAGGTGTGG(+ACTCAC)GATCCCGATCCTGAGCTGTGTCCTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240604220137_dika TGACACCTGATACCAGCAGGAGGTATACTTGG(+ACTCAC)GATCCAATATGAATCAGTGGATTGCTCTGGT NGG 10 8 17 8 24 0 100 5 10
    240604220136_25rs CTAGAACCAGAGCAATCCACTGATTCATATTG(+ACTCAC)GATCCCAAGTATACCTCCTGCTGGTATCAGG NGG 10 8 17 8 24 0 100 5 10
    240604220134_e852 AACTGCAGACTGGAGAGACCTGCACTTCTGGG(+ACTCAC)GATCCTGCACCGACCTACAGCTTCCTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604220133_3aqn AATTTCTTCAAGGAAGCTGTAGGTCGGTGCAG(+ACTCAC)GATCCCCAGAAGTGCAGGTCTCTCCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604220130_uyhf AGACTCTTCCAGGGGAGGGGTGCCAGGATGGG(+ACTCAC)GTACCCCTGGTCACTCACCAGGATCACTGTA NGG 10 8 17 8 24 0 100 5 10
    240604220128_uaa8 AGGGTTACAGTGATCCTGGTGAGTGACCAGGG(+ACTCAC)GTACCCCATCCTGGCACCCCTCCCCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604220125_lztu TGGTTACCTTGCCCTGAAAGATGCAGCCAGGG(+ACTCAC)GTACCTGGGTTAACCCAAGGGCCCAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240604220123_eho1 AGACCAGGGCTTGGGCCCTTGGGTTAACCCAG(+ACTCAC)GTACCCCTGGCTGCATCTTTCAGGGCAAGGT NGG 10 8 17 8 24 0 100 5 10