Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220121_q3fk TGGGTGAGGAGGTACACACAGGGCTCAGGATG(+ACTCAC)GATCCCCTATGGGGAGGAGGGCAGACAATTT NGG 10 8 17 8 24 0 100 5 10
    240604220119_bs4y AGTGCAAATTGTCTGCCCTCCTCCCCATAGGG(+ACTCAC)GATCCATCCTGAGCCCTGTGTGTACCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240604220117_p9j9 TGGGAATCACACTTTAAAAGGGTCAGAATATG(+ACTCAC)GATCCCGAGGGAAAGAATGCTCCGCACTGTT NGG 10 8 17 8 24 0 100 5 10
    240604220115_l5j4 TCATGAACAGTGCGGAGCATTCTTTCCCTCGG(+ACTCAC)GATCCATATTCTGACCCTTTTAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240604220114_c8p5 TGCAATGCCTCAGAGACAAGAAGAGTGCATTG(+ACTCAC)GATCCTAGGGGGCTGCAGTTACAGTCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240604220111_t0ia ACTCACAACAGACTGTAACTGCAGCCCCCTAG(+ACTCAC)GATCCAATGCACTCTTCTTGTCTCTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604220108_kv27 TGCAATATTTGTACACATATACAAGTCCTGAG(+ACTCAC)GATCCTAATTTCAGCTCAGAGATCGAATTGG NGG 10 8 17 8 24 0 100 5 10
    240604220107_w048 CTGACCCAATTCGATCTCTGAGCTGAAATTAG(+ACTCAC)GATCCTCAGGACTTGTATATGTGTACAAATA NGG 10 8 17 8 24 0 100 5 10
    240604220105_nk0l CAAGGAGTTCTAGCATACACTTTTGTGATTGG(+ACTCAC)GTACCATATGGTTATATCTGATTCTTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240604220103_9yjl TCATGCTACCAAGAATCAGATATAACCATATG(+ACTCAC)GTACCCAATCACAAAAGTGTATGCTAGAACT NGG 10 8 17 8 24 0 100 5 10