Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220047_ji4o ATATCATAGACTATAATAGAAAACAACATTTG(+ACTCAC)GATCCATTGGAAGTGCAAAGATAAAGTATTT NGG 10 8 17 8 24 0 100 5 10
    240604220045_r4a3 CATGTAAATACTTTATCTTTGCACTTCCAATG(+ACTCAC)GATCCAAATGTTGTTTTCTATTATAGTCTAT NGG 10 8 17 8 24 0 100 5 10
    240604220043_12pr AAGTTTTTCATTGTTGAAAGGGTGGCAGAGCG(+ACTCAC)GATCCAGATACCTGAGATCCTGGTAATTGTT NGG 10 8 17 8 24 0 100 5 10
    240604220042_2qum ACGTCAACAATTACCAGGATCTCAGGTATCTG(+ACTCAC)GATCCGCTCTGCCACCCTTTCAACAATGAAA NGG 10 8 17 8 24 0 100 5 10
    240604220040_cy7d GCTTTATTTATGTTCCTGCCCCTAAATTTTAG(+ACTCAC)GTACCATAATTAATATTAGGAAATGTCACTG NGG 10 8 17 8 24 0 100 5 10
    240604220039_eix6 AAGGGCAGTGACATTTCCTAATATTAATTATG(+ACTCAC)GTACCTAAAATTTAGGGGCAGGAACATAAAT NGG 10 8 17 8 24 0 100 5 10
    240604220038_gpdw TCATCAATGTTCCTACAGAAGACCTGGGCTTG(+ACTCAC)GTACCCAGCTTCTATACGTCTGCTCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240604220036_k1vc TATGAGGCTGTGAGCAGACGTATAGAAGCTGG(+ACTCAC)GTACCAAGCCCAGGTCTTCTGTAGGAACATT NGG 10 8 17 8 24 0 100 5 10
    240604220034_h9m9 GGCTGGAACATTCACTTTGTACAGTACCATTG(+ACTCAC)GTACCAGCAAGAAATATCCACAGGAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240604220032_zdmm ATTTCATTTATTCCTGTGGATATTTCTTGCTG(+ACTCAC)GTACCAATGGTACTGTACAAAGTGAATGTTC NGG 10 8 17 8 24 0 100 5 10