Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214758_pwhy GGCAGAGATGGCTCCATGTTTTACAAACTATG(+ACTCAC)GATCCCTGGGCAGAGTTCAGGTGTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604214755_wq3e AAGGATACAGGGACACCTGAACTCTGCCCAGG(+ACTCAC)GATCCATAGTTTGTAAAACATGGAGCCATCT NGG 10 8 17 8 24 0 100 5 10
    240604214754_kfq9 GAGGCATGTCAGCTACCAGTTTCTCCACAGAG(+ACTCAC)GATCCAAGAAGTACATCAAAGATGGTCTACC NGG 10 8 17 8 24 0 100 5 10
    240604214753_2nba GAGATGGTAGACCATCTTTGATGTACTTCTTG(+ACTCAC)GATCCTCTGTGGAGAAACTGGTAGCTGACAT NGG 10 8 17 8 24 0 100 5 10
    240604214751_35jw CAGTGAGTGCCAGCGGTGCTGGAGCTAGAGCG(+ACTCAC)GATCCTGAAGGCAGTGACATTGGGACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240604214750_aina GACCCTGACTGTCCCAATGTCACTGCCTTCAG(+ACTCAC)GATCCGCTCTAGCTCCAGCACCGCTGGCACT NGG 10 8 17 8 24 0 100 5 10
    240604214749_3mk7 TAAGAAGAGTCTCCCCTGAAGTGTTTGTGGTG(+ACTCAC)GTACCTGTGTGCAGAAGGGTGTGTCGCCACT NGG 10 8 17 8 24 0 100 5 10
    240604214747_ydfg TGCCAAGTGGCGACACACCCTTCTGCACACAG(+ACTCAC)GTACCACCACAAACACTTCAGGGGAGACTCT NGG 10 8 17 8 24 0 100 5 10
    240604214746_h2so TGAGATGTCCTGACTAATCTTCCAGAACACAG(+ACTCAC)GATCCAGCAGGGAAAAGATTCACCAGCTAAT NGG 10 8 17 8 24 0 100 5 10
    240604214745_4wkk AAGAAATTAGCTGGTGAATCTTTTCCCTGCTG(+ACTCAC)GATCCTGTGTTCTGGAAGATTAGTCAGGACA NGG 10 8 17 8 24 0 100 5 10