Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214651_h9zf AAGCCAACAAACTGAGAATTTGCCCCTGTAAG(+ACTCAC)GATCCATGTGGTAGATACTAGCTGCTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604214649_6zcw TTGGCCAAAGAGCAGCTAGTATCTACCACATG(+ACTCAC)GATCCTTACAGGGGCAAATTCTCAGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240604214648_a5wx TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+ACTCAC)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604214646_or6b GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+ACTCAC)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240604214644_vqp0 CCACATGGTGGTTCACAACCATCTGTAATGGG(+ACTCAC)GATCCATATACATAAATAAACCTTTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604214643_rmk6 AATATTTTTTAAAAGGTTTATTTATGTATATG(+ACTCAC)GATCCCCATTACAGATGGTTGTGAACCACCA NGG 10 8 17 8 24 0 100 5 10
    240604214641_du3p TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+ACTCAC)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604214640_4i1w GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+ACTCAC)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240604214638_d6is CTAGGGAGCCATCTGGGATGTTGGCATGTTGG(+ACTCAC)GTACCTGTCCTGTGACACTTGATTGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240604214637_ea5x CCACCCAAGCACAATCAAGTGTCACAGGACAG(+ACTCAC)GTACCCAACATGCCAACATCCCAGATGGCTC NGG 10 8 17 8 24 0 100 5 10