Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214537_frs2 AAGGGGCTCTACCACTTGGGACTCTAGGATCG(+ACTCAC)GTACCTGCCATGGTGGGGAAGTTAGGGATAG NGG 10 8 17 8 24 0 100 5 10
    240604214535_ng8h CTAGCCTATCCCTAACTTCCCCACCATGGCAG(+ACTCAC)GTACCGATCCTAGAGTCCCAAGTGGTAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604214534_dtb2 CTTAGTCCTTAGACTTGATGTGCCATGGATTG(+ACTCAC)GTACCATGGGTGGTAGTGGGGGTGGTGACAG NGG 10 8 17 8 24 0 100 5 10
    240604214530_2wnm GAGCCCTGTCACCACCCCCACTACCACCCATG(+ACTCAC)GTACCAATCCATGGCACATCAAGTCTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604214528_01p9 CAGTCAGGGACAAGGCTGTCTTCAGGTGTGGG(+ACTCAC)GATCCTGGGACTCCAACATGCAGGAAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604214526_6zbi GTAGAGCCCCTTCCTGCATGTTGGAGTCCCAG(+ACTCAC)GATCCCCACACCTGAAGACAGCCTTGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240604214524_sukd GCTCACAATGGCCTACAACTCCAGTTCCTGAG(+ACTCAC)GATCCAGTACCCTTACATTCACACACATGAA NGG 10 8 17 8 24 0 100 5 10
    240604214522_e2w6 TTGCCTTCATGTGTGTGAATGTAAGGGTACTG(+ACTCAC)GATCCTCAGGAACTGGAGTTGTAGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240604214520_13z0 CTTATCTTCAGACACACTAGAAGAGGCCATCG(+ACTCAC)GATCCTATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604214519_pbr5 ATCACATGGTGGCTCACAACCATCTGTAATAG(+ACTCAC)GATCCGATGGCCTCTTCTAGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10