Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214500_bd5r ACACTGAGAGAGCAGCAATAACTATCACCTCG(+ACTCAC)GATCCAGAGTAACACTGAAGTACACGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240604214459_8jrq GGCTGGCCTCCGTGTACTTCAGTGTTACTCTG(+ACTCAC)GATCCGAGGTGATAGTTATTGCTGCTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240604214458_djke TTTAACTTAACTGGGGTTGACAATTCAAATAG(+ACTCAC)GTACCTCCCCCCACCCCCCCAAACAAAAACC NGG 10 8 17 8 24 0 100 5 10
    240604214456_17bk AGTCAGGTTTTTGTTTGGGGGGGTGGGGGGAG(+ACTCAC)GTACCTATTTGAATTGTCAACCCCAGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240604214455_90sj ACCTGCCCTCAATCCTAGAGGGAAGACTGAAG(+ACTCAC)GTACCCTTGGGGCTGGAATCTTGAGACAGCC NGG 10 8 17 8 24 0 100 5 10
    240604214452_pd4t CCACAGGCTGTCTCAAGATTCCAGCCCCAAGG(+ACTCAC)GTACCTTCAGTCTTCCCTCTAGGATTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240604214450_7w78 GGTCCCTTCACACTATGTCCACATTTACCTGG(+ACTCAC)GTACCACATGAAAGATGGAGGAGGCGGGACT NGG 10 8 17 8 24 0 100 5 10
    240604214448_q3bw GCATTAGTCCCGCCTCCTCCATCTTTCATGTG(+ACTCAC)GTACCCAGGTAAATGTGGACATAGTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604214447_4re5 ATCAAGCAATCATCCCAAAAGGTAGCTCTGTG(+ACTCAC)GTACCTCTGTTGACCCTGGCTGTGACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604214446_hvat GACAGCCCAGGTCACAGCCAGGGTCAACAGAG(+ACTCAC)GTACCACAGAGCTACCTTTTGGGATGATTGC NGG 10 8 17 8 24 0 100 5 10