Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214408_zk4v TTACTCACAAATAAACTAATGCTACTTGCAAG(+ACTCAC)GATCCTTGGTAAAGTAAAACCTGATGAATAT NGG 10 8 17 8 24 0 100 5 10
    240604214406_wv77 TGAAAATATTCATCAGGTTTTACTTTACCAAG(+ACTCAC)GATCCTTGCAAGTAGCATTAGTTTATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240604214404_ai7m GGAGAGGATAACCAAAGCCTGGCTACAACTGG(+ACTCAC)GTACCAGAATGCCTGCCGACCACCTGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240604214403_fabm GGTGAGGCAGCAGGTGGTCGGCAGGCATTCTG(+ACTCAC)GTACCCAGTTGTAGCCAGGCTTTGGTTATCC NGG 10 8 17 8 24 0 100 5 10
    240604214401_1z86 TGAACTCTTGGTTTGGGAAAGAGGAGAGTCAG(+ACTCAC)GATCCTCCACTCAGGGGCCTGCTTGGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240604214400_z619 AGGGCTGCAGCCAAGCAGGCCCCTGAGTGGAG(+ACTCAC)GATCCTGACTCTCCTCTTTCCCAAACCAAGA NGG 10 8 17 8 24 0 100 5 10
    240604214357_a3di GTTACCTTTCGCTGTGGGGAGGCCGCTGCCGG(+ACTCAC)GATCCGGCCCCGAACAGCCCAGGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240604214355_7tr6 CCCCGCCCCTGCCCCTGGGCTGTTCGGGGCCG(+ACTCAC)GATCCCGGCAGCGGCCTCCCCACAGCGAAAG NGG 10 8 17 8 24 0 100 5 10
    240604214354_dlss CGGGGTCCTGCCCTACCCAGGCTATGCTACAG(+ACTCAC)GATCCACCACAGTCCAAAACCAGGAATTAGA NGG 10 8 17 8 24 0 100 5 10
    240604214352_u2v6 CTCTCTCTAATTCCTGGTTTTGGACTGTGGTG(+ACTCAC)GATCCTGTAGCATAGCCTGGGTAGGGCAGGA NGG 10 8 17 8 24 0 100 5 10