Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214516_f69f CCCTTCCCACTGAGATGCCTGGGCAGCCATGG(+ACTCAC)GTACCCCGAGGCTGATAACACATCAGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240604214514_nejc AATAGCACCTCTGATGTGTTATCAGCCTCGGG(+ACTCAC)GTACCCATGGCTGCCCAGGCATCTCAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240604214513_3ai7 GAACCCAGAAGGAGGGTCCAGTGGGCTCTTTG(+ACTCAC)GATCCTTCAGGCTCTTGGAGACGAGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604214512_3613 CTCTCCAGCTTCTCGTCTCCAAGAGCCTGAAG(+ACTCAC)GATCCAAAGAGCCCACTGGACCCTCCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240604214509_qxhi CTACATTCAGGAATATATATACACATTCCTGG(+ACTCAC)GTACCTAGCCACACATGCATACATAAACACA NGG 10 8 17 8 24 0 100 5 10
    240604214508_sa2k GCATGTGTGTTTATGTATGCATGTGTGGCTAG(+ACTCAC)GTACCCAGGAATGTGTATATATATTCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240604214506_tp5n CTCCTCCTTTGAGTCATTGCTTCACCCACTAG(+ACTCAC)GATCCTGCCTTACCTTGACACCCAGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240604214505_0xve TTGTTTTGTTTCTGGGTGTCAAGGTAAGGCAG(+ACTCAC)GATCCTAGTGGGTGAAGCAATGACTCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240604214504_fl64 AGAAAGGGAGGGATAACCTCTTTCTCAACTAG(+ACTCAC)GTACCAGCTTGAGTCCTTATGTCAAAGGCGA NGG 10 8 17 8 24 0 100 5 10
    240604214502_mcsi CTTCATCGCCTTTGACATAAGGACTCAAGCTG(+ACTCAC)GTACCTAGTTGAGAAAGAGGTTATCCCTCCC NGG 10 8 17 8 24 0 100 5 10