Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214635_7ktl GGAGAGTCACTGGCCTCTCCCGTTGGTTACAG(+ACTCAC)GTACCCTTATACCCTGGATGTTAGCGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240604214634_6ax8 ACAACTACAACGCTAACATCCAGGGTATAAGG(+ACTCAC)GTACCTGTAACCAACGGGAGAGGCCAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240604214631_cjlw CTTGAAAAGCTGGCAGCACTGCAGCCTCCCTG(+ACTCAC)GTACCATTGGTCTGACAGACACAGTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604214629_2yej GACTATCTGGAACTGTGTCTGTCAGACCAATG(+ACTCAC)GTACCAGGGAGGCTGCAGTGCTGCCAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604214627_vy74 CGGAGGATGAAGCCCATCCCTTGCAGATGGGG(+ACTCAC)GTACCCATGCTTTGATGATGTTCACAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240604214625_5mod AGAAGCCCTCTGTGAACATCATCAAAGCATGG(+ACTCAC)GTACCCCCATCTGCAAGGGATGGGCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240604214623_jy8p TCGTGTCTATCCAGGCTGGGGGCCAGACTGGG(+ACTCAC)GATCCCTTTCTGGGGTCTGCACTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240604214622_esqx CACACACACACACAGTGCAGACCCCAGAAAGG(+ACTCAC)GATCCCCAGTCTGGCCCCCAGCCTGGATAGA NGG 10 8 17 8 24 0 100 5 10
    240604214619_k76z TGACTTTTCATGGGCGTGCTCTAGGAATCCTG(+ACTCAC)GATCCCACTAAACTTCCATGGCTGGTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240604214617_8gto CCCGTGCCCAACCAGCCATGGAAGTTTAGTGG(+ACTCAC)GATCCAGGATTCCTAGAGCACGCCCATGAAA NGG 10 8 17 8 24 0 100 5 10