Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214724_77td CGTGCCATCCCCATCAGGACCTCAGACCTGGG(+ACTCAC)GTACCTGAACTCTGTCCCTCTGCTGCCGCCT NGG 10 8 17 8 24 0 100 5 10
    240604214723_ibx4 GACAGAGGCGGCAGCAGAGGGACAGAGTTCAG(+ACTCAC)GTACCCCAGGTCTGAGGTCCTGATGGGGATG NGG 10 8 17 8 24 0 100 5 10
    240604214721_k0yl TCCTCACTCATTTTCAACGCTGACCCTGACAG(+ACTCAC)GATCCACAGACTTCTCCAGGACCTTCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604214720_ii3h AGGGTCTCTGGAAGGTCCTGGAGAAGTCTGTG(+ACTCAC)GATCCTGTCAGGGTCAGCGTTGAAAATGAGT NGG 10 8 17 8 24 0 100 5 10
    240604214718_gj7s TCCCCCAGGGGAGAGGCCCCTTTACCTCAGTG(+ACTCAC)GATCCTGTTAAGGAGTGCTTCCAGCCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240604214717_8y9b CCCAGGCTGAGGCTGGAAGCACTCCTTAACAG(+ACTCAC)GATCCACTGAGGTAAAGGGGCCTCTCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604214715_phpp CCTACAGTGTTACTTCCTAGGGGCTACGCAGG(+ACTCAC)GATCCGTTTCTCTCCACTGTGCTATTCACAA NGG 10 8 17 8 24 0 100 5 10
    240604214713_qrn3 CAACGTTGTGAATAGCACAGTGGAGAGAAACG(+ACTCAC)GATCCCTGCGTAGCCCCTAGGAAGTAACACT NGG 10 8 17 8 24 0 100 5 10
    240604214711_aup5 AGAAGAAAGCAATCCCTGAAATCTGGCAGGCG(+ACTCAC)GTACCAGAGGCCACAGTTGTTCTGGTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240604214709_ybju TACCACACTCACCAGAACAACTGTGGCCTCTG(+ACTCAC)GTACCGCCTGCCAGATTTCAGGGATTGCTTT NGG 10 8 17 8 24 0 100 5 10