Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214351_2i73 AGATGTATCCTGAAGCCTTCTTGCCTATCTGG(+ACTCAC)GTACCAGGTGTGACCTCAAGAGATGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240604214349_m202 TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+ACTCAC)GTACCCAGATAGGCAAGAAGGCTTCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240604214347_thwe GGTGACCTCAAAGGTATCCCATGATCAGCATG(+ACTCAC)GTACCAGCAGTAGTGAGTAACCAGTTCTAGT NGG 10 8 17 8 24 0 100 5 10
    240604214346_8bz3 GGGAAACTAGAACTGGTTACTCACTACTGCTG(+ACTCAC)GTACCATGCTGATCATGGGATACCTTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240604214345_dess ACGTGATGGTAGGCTTGTGGGGTGACTGCTAG(+ACTCAC)GTACCTCTAGAGGGCAACACTTACACAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604214344_lvau CTGTTTTCCTGTGTAAGTGTTGCCCTCTAGAG(+ACTCAC)GTACCTAGCAGTCACCCCACAAGCCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240604214341_86ai CAGGTGGTCTGACATCCTCTTGTGCCTCCCTG(+ACTCAC)GTACCTGCAGACACATTCTCTCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240604214340_0xly GAGAGAGACAGAGAGAGAGAATGTGTCTGCAG(+ACTCAC)GTACCAGGGAGGCACAAGAGGATGTCAGACC NGG 10 8 17 8 24 0 100 5 10
    240604214338_nelm GTGTGCACTGCTTGTATGCGTGTGGATGTGTG(+ACTCAC)GATCCCACCTGCCGCCTCCCCATCTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240604214337_84ce ACACCCAGAGCAGATGGGGAGGCGGCAGGTGG(+ACTCAC)GATCCACACATCCACACGCATACAAGCAGTG NGG 10 8 17 8 24 0 100 5 10