Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214238_l8m6 TCTGGATGTCACCATGTGTTTCTCAAAATCTG(+ACTCAC)GTACCCTCCCCTCTCAGAGATTACTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240604214236_cjsc TGGCAGAAGTCAGTAATCTCTGAGAGGGGAGG(+ACTCAC)GTACCAGATTTTGAGAAACACATGGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240604214234_hs8g TTCCTCTTCTCCTCCCCATGGAAGGGGGGGGG(+ACTCAC)GATCCCTGGCTCAGCATCCACTGCTGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604214231_ctbf AGGTTTCCACCAGCAGTGGATGCTGAGCCAGG(+ACTCAC)GATCCCCCCCCCCTTCCATGGGGAGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604214229_xzss TACCTGCCTCTCAAGTGCTGGAATTAAAGGCG(+ACTCAC)GTACCAGCAGCCCGGCTCCTTTGTTCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240604214228_uvay ATAACAAAAGGAACAAAGGAGCCGGGCTGCTG(+ACTCAC)GTACCGCCTTTAATTCCAGCACTTGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240604214226_wm2e ATACCTTCAGAGACAAACATGTTTACACATAG(+ACTCAC)GTACCCGTGTGAGCCTAGATACACACATACA NGG 10 8 17 8 24 0 100 5 10
    240604214225_aiud TTTAGTGTATGTGTGTATCTAGGCTCACACGG(+ACTCAC)GTACCTATGTGTAAACATGTTTGTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240604214224_wu0c AGACTTAAGAGTAGGAAAGGGACTGGTACATG(+ACTCAC)GTACCTGCCAGCCCAGCCTAGCACGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240604214222_m27s AAAAAACTCTTCGTGCTAGGCTGGGCTGGCAG(+ACTCAC)GTACCATGTACCAGTCCCTTTCCTACTCTTA NGG 10 8 17 8 24 0 100 5 10