Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214130_y24f ATAAAGTTCAGTGCTATAGCACTTTGTGTGAG(+ACTCAC)GTACCTGGGTTCAACCCCAGCACTGCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240604214128_e5xn GTGGGATGAGGCAGTGCTGGGGTTGAACCCAG(+ACTCAC)GTACCTCACACAAAGTGCTATAGCACTGAAC NGG 10 8 17 8 24 0 100 5 10
    240604214126_iuco GCCTGCGCTGGAGCAGTGCGAGCTTGTCCGTG(+ACTCAC)GTACCCGTACCCGCCCGCCCGCAGCGTGGCG NGG 10 8 17 8 24 0 100 5 10
    240604214124_zht0 AATTGCGCCACGCTGCGGGCGGGCGGGTACGG(+ACTCAC)GTACCACGGACAAGCTCGCACTGCTCCAGCG NGG 10 8 17 8 24 0 100 5 10
    240604214123_zv1t CTTGGTTTCTCACTCCAGGCATCTTAGACTTG(+ACTCAC)GATCCTATTAAAAATCACATTCCCCAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604214122_11yc AAACCTGCCTTGGGGAATGTGATTTTTAATAG(+ACTCAC)GATCCAAGTCTAAGATGCCTGGAGTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604214120_aeig TTCTCCTCTTTCCTCTCTCTAGGTCCTTCCAG(+ACTCAC)GTACCCTCCCTCCAACCCTTTCCATGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604214118_ikso AGCAGGGGAACATGGAAAGGGTTGGAGGGAGG(+ACTCAC)GTACCTGGAAGGACCTAGAGAGAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240604214115_vg28 GCTAGGGAAAAGGAGTGTTCGTTCTACCCAGG(+ACTCAC)GTACCACAGTTCCCCACAGTCAAAACCCAAA NGG 10 8 17 8 24 0 100 5 10
    240604214114_jauw TTGCTTTTGGGTTTTGACTGTGGGGAACTGTG(+ACTCAC)GTACCCTGGGTAGAACGAACACTCCTTTTCC NGG 10 8 17 8 24 0 100 5 10