Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214038_yj81 TTCCACAAAGTATAGACAAAGACCCCTACCAG(+ACTCAC)GTACCTTTGTGTGCTCATGTAGTAGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240604214037_yfsv TGTGACAGTTTCTACTACATGAGCACACAAAG(+ACTCAC)GTACCTGGTAGGGGTCTTTGTCTATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240604214035_bxt1 ACCCAGTAGCACCCGACTGTAGTGTGATTGGG(+ACTCAC)GTACCCTCTCCAGATTCTCTATCTTAACCAC NGG 10 8 17 8 24 0 100 5 10
    240604214033_qiia ACAAGGTGGTTAAGATAGAGAATCTGGAGAGG(+ACTCAC)GTACCCCAATCACACTACAGTCGGGTGCTAC NGG 10 8 17 8 24 0 100 5 10
    240604214031_9vx2 TTCTGCCCCCTTTGCCTGACCCCCAAGCCTGG(+ACTCAC)GTACCGCAAACACCAAGGCCTCCAGACAGTG NGG 10 8 17 8 24 0 100 5 10
    240604214029_b81d CCCTGCACTGTCTGGAGGCCTTGGTGTTTGCG(+ACTCAC)GTACCCAGGCTTGGGGGTCAGGCAAAGGGGG NGG 10 8 17 8 24 0 100 5 10
    240604214027_k6tq AGACAATTCCTGTTCAGAGACAACAGGGGCCG(+ACTCAC)GTACCACTGGGAGATGGCAAAAGTTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604214025_zarc GGGTGGGAAGAAACTTTTGCCATCTCCCAGTG(+ACTCAC)GTACCGGCCCCTGTTGTCTCTGAACAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240604214023_r2lw GCAAGAGTCGGTTCCCTCCTTCTACTGTGTAG(+ACTCAC)GTACCAGTGATTAAACAGGCCGTTGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240604214022_kjal GCCTCCGAGCTCAACGGCCTGTTTAATCACTG(+ACTCAC)GTACCTACACAGTAGAAGGAGGGAACCGACT NGG 10 8 17 8 24 0 100 5 10