Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213948_ghyj GTTTTGATTCTCATGAGTAAAGAAGAAACAAG(+ACTCAC)GTACCCACCCCTGGATGCTGGACGAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604213946_18gq AGGGGCCTCTCTCGTCCAGCATCCAGGGGTGG(+ACTCAC)GTACCTTGTTTCTTCTTTACTCATGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240604213945_s4yj TCCCATTAAGGAAAATGTGAAAATTGCATATG(+ACTCAC)GATCCACTCTCACCATAAAGGTGAGAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604213943_adhy CCTGCCTTCTTCTCACCTTTATGGTGAGAGTG(+ACTCAC)GATCCATATGCAATTTTCACATTTTCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240604213942_i6mz AGTGTCTGGGTTTGCTGTCTGCAAGTGGAATG(+ACTCAC)GATCCCAAGGTGGGCCAGTGTCTTGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240604213940_6f6f GGAAAGGCCATCAAGACACTGGCCCACCTTGG(+ACTCAC)GATCCATTCCACTTGCAGACAGCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240604213939_h6c2 GAAAAACTGTTTATAAGAACCAAAATGATGTG(+ACTCAC)GATCCCTGTGTGTCCTGTGGAGGTGACATCA NGG 10 8 17 8 24 0 100 5 10
    240604213937_22cz GAACTTGATGTCACCTCCACAGGACACACAGG(+ACTCAC)GATCCACATCATTTTGGTTCTTATAAACAGT NGG 10 8 17 8 24 0 100 5 10
    240604213936_t93y ATCAACAGCAAACTTAAAGGAAAAAGGAGCTG(+ACTCAC)GTACCCCAATGTCCTGACCTGGAGTCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240604213934_eeu2 GCCAAATCTTGACTCCAGGTCAGGACATTGGG(+ACTCAC)GTACCAGCTCCTTTTTCCTTTAAGTTTGCTG NGG 10 8 17 8 24 0 100 5 10