Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213846_63mt GAGTGAAAACAAGCTTTTCTTCAAATCTTCAG(+ACTCAC)GTACCTACCTCAGAATAGGTGTCATTAACGT NGG 10 8 17 8 24 0 100 5 10
    240604213844_oevo TGAAAACGTTAATGACACCTATTCTGAGGTAG(+ACTCAC)GTACCTGAAGATTTGAAGAAAAGCTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240604213843_76jz CTCGAGGAATAGAAACACTGGATACTTAATGG(+ACTCAC)GTACCAGAAGAGTTTAGGAAACATGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240604213841_jhf9 AGCCACTCCTCCATGTTTCCTAAACTCTTCTG(+ACTCAC)GTACCCATTAAGTATCCAGTGTTTCTATTCC NGG 10 8 17 8 24 0 100 5 10
    240604213840_3vjm TGGCAGTTTGATGGCAGTACTCTGTGTAGCAG(+ACTCAC)GATCCTGGATGAGACGAGGAGGAATCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240604213838_u90q CATGGCTGCAGATTCCTCCTCGTCTCATCCAG(+ACTCAC)GATCCTGCTACACAGAGTACTGCCATCAAAC NGG 10 8 17 8 24 0 100 5 10
    240604213836_kvh2 GAGCTTAAAAGACACTCGTTAGAGTGCACCTG(+ACTCAC)GATCCTGCCATGACATCACAGGCGAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240604213835_5i6f CGGCTGATGCTTCGCCTGTGATGTCATGGCAG(+ACTCAC)GATCCAGGTGCACTCTAACGAGTGTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240604213833_owjw AAATGACCAGGCTCCTGGTAAATGGAAATCAG(+ACTCAC)GATCCAAGCAACTTTGAATTCTGGGAAATGA NGG 10 8 17 8 24 0 100 5 10
    240604213832_i6mn CAAAGTCATTTCCCAGAATTCAAAGTTGCTTG(+ACTCAC)GATCCTGATTTCCATTTACCAGGAGCCTGGT NGG 10 8 17 8 24 0 100 5 10