Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213932_freu GCTACCTTTTAGGACTGAAGTTTTAAGCACTG(+ACTCAC)GTACCCACTGTGGGGCAAACCTGTGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240604213931_763e CCCTCCTGCAGCACAGGTTTGCCCCACAGTGG(+ACTCAC)GTACCAGTGCTTAAAACTTCAGTCCTAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604213929_cky0 GGTGAGCTCTGTACAGTAATGGTGGTGTCCAG(+ACTCAC)GTACCGCTAGGAAACACGGGAGTCCGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604213927_v47v AGCCGCTGGACGGACTCCCGTGTTTCCTAGCG(+ACTCAC)GTACCTGGACACCACCATTACTGTACAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604213926_due6 GAAGCCCCGTGGAAGCCAAAAGCAGTTTGGTG(+ACTCAC)GATCCGTGGCAGCAGCTTGGAGCTGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240604213924_28rn TTTTGAAATCACAGCTCCAAGCTGCTGCCACG(+ACTCAC)GATCCACCAAACTGCTTTTGGCTTCCACGGG NGG 10 8 17 8 24 0 100 5 10
    240604213922_zly2 GAGGGACCATTTTCCTGACTGTACCTCTTTGG(+ACTCAC)GTACCCCATTCCTAAAGCAATAACTCAACGG NGG 10 8 17 8 24 0 100 5 10
    240604213920_7xo1 CATTACCGTTGAGTTATTGCTTTAGGAATGGG(+ACTCAC)GTACCCAAAGAGGTACAGTCAGGAAAATGGT NGG 10 8 17 8 24 0 100 5 10
    240604213919_rl06 CGTCCCTAGAAGCACAGGGCCCCTAGAAGCAG(+ACTCAC)GTACCAGCTCTGAGAACTTCCTGGCTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240604213918_0iuq TGCACCAAGGAGCCAGGAAGTTCTCAGAGCTG(+ACTCAC)GTACCTGCTTCTAGGGGCCCTGTGCTTCTAG NGG 10 8 17 8 24 0 100 5 10