Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211109_78nb ACATTAGAAGAAGATGTGATGGAGGCCATGGG(+ACTCAC)GATCCAGGAGACTCGAAGATTCAAGAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240604211107_1xzu TTAGATCGATTTTTTTTAAAAGTATGTGTTGG(+ACTCAC)GTACCTTTTCTGTGTGTATGCTCAGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604211105_42q5 ACACAGCCTGCCTGAGCATACACACAGAAAAG(+ACTCAC)GTACCCAACACATACTTTTAAAAAAAATCGA NGG 10 8 17 8 24 0 100 5 10
    240604211104_2xqo TCTTCTCCACGTACCCACAGAAGGCAGAAGTG(+ACTCAC)GTACCAGGCAAGAAGATGGGATTGTTGCATT NGG 10 8 17 8 24 0 100 5 10
    240604211102_rvql AACAAAATGCAACAATCCCATCTTCTTGCCTG(+ACTCAC)GTACCACTTCTGCCTTCTGTGGGTACGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604211100_nrur ATGGTATATAAAGAGTGTACCTTATAAGCCAG(+ACTCAC)GTACCTTTAATCCCAGAACTTGGGTTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604211059_34kc GCTGGCTTCAAACCCAAGTTCTGGGATTAAAG(+ACTCAC)GTACCTGGCTTATAAGGTACACTCTTTATAT NGG 10 8 17 8 24 0 100 5 10
    240604211057_2m5f GAATAAAAGAATAATATATAAAATTAATAAAG(+ACTCAC)GTACCAAATGCATCTATAAAAAATGGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240604211056_ddw2 TCTCAAGGAACCATTTTTTATAGATGCATTTG(+ACTCAC)GTACCTTTATTAATTTTATATATTATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240604211055_m9qe ATCTGTTCTATGGAATAAGAAAGGGAGGCGTG(+ACTCAC)GTACCCAAAGCATCCTGTAAAGCCAGCATTA NGG 10 8 17 8 24 0 100 5 10