Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211038_4ufk AGTGGGTCCATGCTGAGACATCTTTCCCTGAG(+ACTCAC)GTACCATGATGGGTCTAGTTTAAAATAAACC NGG 10 8 17 8 24 0 100 5 10
    240604211037_2e7q TACCCAGGGAGATGCGAGGAGATAGATTCATG(+ACTCAC)GTACCTGAGCACAGGTAACCAGCCACATGGT NGG 10 8 17 8 24 0 100 5 10
    240604211035_q2qt ATTCTACCATGTGGCTGGTTACCTGTGCTCAG(+ACTCAC)GTACCATGAATCTATCTCCTCGCATCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240604211034_vpl3 GGCTAACTGTAGCTGTATGTTAACAGTGGCAG(+ACTCAC)GTACCCAGCTCCTCTCCCTGAGGCTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240604211032_o7kf ATGGCAGCTGTAGCCTCAGGGAGAGGAGCTGG(+ACTCAC)GTACCTGCCACTGTTAACATACAGCTACAGT NGG 10 8 17 8 24 0 100 5 10
    240604211031_3pn7 TAGTAGGAGTGGGAAATGGATTACTTTGTCTG(+ACTCAC)GATCCCGAGTGGCTTTAGATTACACTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240604211029_hayy ATGTCCCCTGAGTGTAATCTAAAGCCACTCGG(+ACTCAC)GATCCAGACAAAGTAATCCATTTCCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240604211027_jb12 TGGCAGAGCCTCTCAGGAGACAGCTATATCGG(+ACTCAC)GATCCTGTTAGCAAACACTTCTTGGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240604211025_1ucd TATTGTGGATGCCAAGAAGTGTTTGCTAACAG(+ACTCAC)GATCCCGATATAGCTGTCTCCTGAGAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240604211024_v92z GGCAACTAGCTTACAGTGAAGAGGAGGCTGAG(+ACTCAC)GATCCAAGCTCAGGCACTCTAGGCTACAAAA NGG 10 8 17 8 24 0 100 5 10