Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211506_vmdi AGATCATGTCTCCACTTACTGTCACTCCTAAG(+ACTCAC)GATCCCCTGAAGGTCTTGGAGCTTCACATCC NGG 10 8 17 8 24 0 100 5 10
    240604211504_olh8 TCCAGGGATGTGAAGCTCCAAGACCTTCAGGG(+ACTCAC)GATCCTTAGGAGTGACAGTAAGTGGAGACAT NGG 10 8 17 8 24 0 100 5 10
    240604211502_dmsx CGATCTATGACAATGTTTGGAACTCTCTTTTG(+ACTCAC)GATCCACAAAGCTCCTGCAATCAGCTCCAAT NGG 10 8 17 8 24 0 100 5 10
    240604211501_zm7a GGGTTATTGGAGCTGATTGCAGGAGCTTTGTG(+ACTCAC)GATCCAAAAGAGAGTTCCAAACATTGTCATA NGG 10 8 17 8 24 0 100 5 10
    240604211500_n804 ACTACTCTTGGCTTATATTAGACAGTAGAGTG(+ACTCAC)GTACCCCTACCCCATGGTACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240604211458_ouxe GCATGTGTGTGTGTGTGTACCATGGGGTAGGG(+ACTCAC)GTACCACTCTACTGTCTAATATAAGCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240604211457_km5z TACATTCAAGGACAGCCTGGGCTACAGGATAG(+ACTCAC)GATCCTATCTAAAAACAAAACAAAAATAACC NGG 10 8 17 8 24 0 100 5 10
    240604211455_mcv4 TTGAGGGTTATTTTTGTTTTGTTTTTAGATAG(+ACTCAC)GATCCTATCCTGTAGCCCAGGCTGTCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240604211454_7yrp TGGTTTTGTGGGTGATTTTTCCATATTCCTAG(+ACTCAC)GATCCTAGTAGTTTAAGTGTGTAAATGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604211452_ia89 GAAGTCAGACATTTACACACTTAAACTACTAG(+ACTCAC)GATCCTAGGAATATGGAAAAATCACCCACAA NGG 10 8 17 8 24 0 100 5 10