Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155454_dtmz CGGGCGAACCCCGTGTGCTGGGTCTGGCCATG(+AGGACA)GTACCAGGACACCACATCGTTTCTATTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613155452_8c3a CCCAAGCCGGTTCCTTACCGCGGCCTGAGCCG(+AGGACA)GATCCTACCATCCCTTTGATGGAACAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613155451_ulcd CACATTCAGCTGTTCCATCAAAGGGATGGTAG(+AGGACA)GATCCGGCTCAGGCCGCGGTAAGGAACCGGC NGG 10 8 17 8 24 0 100 5 10
    240613155449_u6jd TCAAGACATTGTAGTGAGGGACCCACAAATGG(+AGGACA)GTACCTTTTTTTTTCCAGTAGGCCTTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240613155448_aa7m TGTTAGTGGCAAGGCCTACTGGAAAAAAAAAG(+AGGACA)GTACCCATTTGTGGGTCCCTCACTACAATGT NGG 10 8 17 8 24 0 100 5 10
    240613155447_ldn2 CCCATGCAACCTAAGAGTCCATAAATTGTTTG(+AGGACA)GATCCATCAATCGTGATATTTAGTAAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240613155445_k0qi ATTTATGAGCTTACTAAATATCACGATTGATG(+AGGACA)GATCCAAACAATTTATGGACTCTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240613155444_n0ep ACATTGTCAACCACAGACCACTGCTAAAATTG(+AGGACA)GATCCTTTGCTTACACCACAGACTCTGAACA NGG 10 8 17 8 24 0 100 5 10
    240613155443_g3rm GCGTTTGTTCAGAGTCTGTGGTGTAAGCAAAG(+AGGACA)GATCCAATTTTAGCAGTGGTCTGTGGTTGAC NGG 10 8 17 8 24 0 100 5 10
    240613155441_d9xs AGTGGTGAAGAGCACTTGTTAGTTTTCCAAAG(+AGGACA)GATCCAGGTTTGATATCCAAGACCTACAGAG NGG 10 8 17 8 24 0 100 5 10