Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155439_wnt1 AGGCTCTCTGTAGGTCTTGGATATCAAACCTG(+AGGACA)GATCCTTTGGAAAACTAACAAGTGCTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613155437_oxty GAGCCCGCGCGCCCCGCTGCCCTCCTCGCCCG(+AGGACA)GATCCCTCTCCTGCCCTCTGACTGGAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613155435_yzxb GTGTAGAGACTCCAGTCAGAGGGCAGGAGAGG(+AGGACA)GATCCGGGCGAGGAGGGCAGCGGGGCGCGCG NGG 10 8 17 8 24 0 100 5 10
    240613155433_1jxx CACCCTGGAGCAGCTAGGGGTTCACGGACCTG(+AGGACA)GATCCTACACAGATCCCAGGTTCCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613155432_pbq6 GATGGGAGGAGGGGAACCTGGGATCTGTGTAG(+AGGACA)GATCCAGGTCCGTGAACCCCTAGCTGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613155430_ry8b CTCTGGGCTCAACAGCCCTGGAGAAGGACGAG(+AGGACA)GATCCCCATCCTCCAGCAAGTCCCAACACAT NGG 10 8 17 8 24 0 100 5 10
    240613155428_vxcn CCAATATGTGTTGGGACTTGCTGGAGGATGGG(+AGGACA)GATCCTCGTCCTTCTCCAGGGCTGTTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613155427_n51n GGGGCACACACACGGACACACACACACACACG(+AGGACA)GATCCAAGGGAGGGGGCTGAAAGATATGGCT NGG 10 8 17 8 24 0 100 5 10
    240613155425_30pb CTCTCAGCCATATCTTTCAGCCCCCTCCCTTG(+AGGACA)GATCCGTGTGTGTGTGTGTGTCCGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240613155423_t90q AGCCCCTGGCACTTGAGGTGTGTGACCATTGG(+AGGACA)GATCCGGATAGCCGCAGCGGGGCTGAGGTCT NGG 10 8 17 8 24 0 100 5 10