Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155349_t465 CTAACTTGGCCAATGATGCCCAAGTTCCTGAG(+AGGACA)GATCCCAAGGCCCTAGGAGCTGACTTAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613155348_2ins AAAGGTTTCAAGGATTTGAGTTCCCTTCACTG(+AGGACA)GATCCCAGAGATGATCATAGTGGCCCATGAT NGG 10 8 17 8 24 0 100 5 10
    240613155347_r5vt CTGCCATCATGGGCCACTATGATCATCTCTGG(+AGGACA)GATCCAGTGAAGGGAACTCAAATCCTTGAAA NGG 10 8 17 8 24 0 100 5 10
    240613155346_5srd ATAAAGGATGCTCAGGAACTGGCATGTAGCTG(+AGGACA)GTACCACCGAGAACTCAATGGGCAGAGTATC NGG 10 8 17 8 24 0 100 5 10
    240613155344_tfb9 TCTTGGATACTCTGCCCATTGAGTTCTCGGTG(+AGGACA)GTACCAGCTACATGCCAGTTCCTGAGCATCC NGG 10 8 17 8 24 0 100 5 10
    240613155343_v6vr ATTTTATCTTGTCCTTGATAATCTGGCTTAAG(+AGGACA)GATCCCTTTAGTATTTACAGAGTACTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613155341_xsvl GTAAGACAACAGTACTCTGTAAATACTAAAGG(+AGGACA)GATCCTTAAGCCAGATTATCAAGGACAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613155340_be34 AATTCTCTGACCTGATCTTTACAGCATCTCTG(+AGGACA)GTACCTCAACCCAGAGCCTGACTCATTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613155339_sgue CACCGTGGGAATGAGTCAGGCTCTGGGTTGAG(+AGGACA)GTACCAGAGATGCTGTAAAGATCAGGTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613155338_2r5c GTGTTAAAGAGTCCTGCATGAGCTGCACTGAG(+AGGACA)GATCCCTAAGTACAATATATCTGATTAGCCT NGG 10 8 17 8 24 0 100 5 10