Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155304_xnfp CTTCAATGGCAGAAATCAATGTGAATTTCTTG(+AGGACA)GATCCAGCAGGTAAAGAATACTCTTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613155302_yhxs CAGCTCACAACCTTTATAAATCCATTTCAGGG(+AGGACA)GATCCAACGCCTCCTTGCGTGTTGCACACAT NGG 10 8 17 8 24 0 100 5 10
    240613155301_j7bi AGCACATGTGTGCAACACGCAAGGAGGCGTTG(+AGGACA)GATCCCCTGAAATGGATTTATAAAGGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613155259_v5m4 TGTGACCAAATACATGGTCAGTTTTGGAGAAG(+AGGACA)GTACCGTGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240613155258_ylga CAAAGAATATACCTTCTTCTCAGCACCTCACG(+AGGACA)GTACCTTCTCCAAAACTGACCATGTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240613155256_6s6p ACAGTTAATCAGCGTTCTACAGCTGTGACTGG(+AGGACA)GTACCTGGCATAAATATCTCTTATTTATTTA NGG 10 8 17 8 24 0 100 5 10
    240613155254_dydf ATAAATAAATAAATAAGAGATATTTATGCCAG(+AGGACA)GTACCCAGTCACAGCTGTAGAACGCTGATTA NGG 10 8 17 8 24 0 100 5 10
    240613155253_qnkm CCAGACCCTGAACCTCCACATGCAGCAGTGTG(+AGGACA)GTACCATGTCAGAGCGCCAGAGCAGAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613155252_bwlh CAGATAGCACTCTGCTCTGGCGCTCTGACATG(+AGGACA)GTACCACACTGCTGCATGTGGAGGTTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613155250_8gdh CATGAAAGAGTTATTAGCCACCAAGTATGCTG(+AGGACA)GTACCTATAATCCCAGAACACAGAAGGCTGA NGG 10 8 17 8 24 0 100 5 10