Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155217_2pd0 CATAGATAACTAACCATACCCTCTTCTACTTG(+AGGACA)GTACCCTCCTAGCTCCCCAGCAAGCCCATCT NGG 10 8 17 8 24 0 100 5 10
    240613155216_3xv6 GTGCTGCCAGGCCAGGCCCTTTCATGACTGTG(+AGGACA)GATCCCCTATGCCTGACAGCCAGAGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613155214_owjy TAGCTGCCTTCCTCTGGCTGTCAGGCATAGGG(+AGGACA)GATCCACAGTCATGAAAGGGCCTGGCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613155210_m7vi TGGAGAGCTGCTGACAGTTGGCAATACCCTGG(+AGGACA)GATCCACATTAGGGACCCCAGTGGACTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613155209_8nfa GGCTCCTCCAGTCCACTGGGGTCCCTAATGTG(+AGGACA)GATCCCAGGGTATTGCCAACTGTCAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613155207_uszi CTGCACATATGTCTGCATCACATGTGTACCTG(+AGGACA)GTACCCATGGACAACATCATAGAACTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613155205_in90 TTGTAACTCCAGTTCTATGATGTTGTCCATGG(+AGGACA)GTACCAGGTACACATGTGATGCAGACATATG NGG 10 8 17 8 24 0 100 5 10
    240613155203_2agf CTGCACATATGTCTGCATCACATGTGTACCTG(+AGGACA)GTACCCATGGACAACATCATAGAACTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613155201_uczx TTGTAACTCCAGTTCTATGATGTTGTCCATGG(+AGGACA)GTACCAGGTACACATGTGATGCAGACATATG NGG 10 8 17 8 24 0 100 5 10
    240613155200_jrvd ATTGTGCATTTGCCATAGGGTCCTTGTTAAAG(+AGGACA)GTACCATAAAAATAATAATTTTGTAATAAAA NGG 10 8 17 8 24 0 100 5 10