Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155114_hydl CACCCAGTCGGGGTGAAGCCCCGCCCCCACGG(+AGGACA)GATCCTCAAGCCCAAATAGGGCCCACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613155112_253u CTCTAGGAATCTCTATGGGGACTGCTAGGAAG(+AGGACA)GATCCTGGGCATGACAGCCTCGTATGATAGC NGG 10 8 17 8 24 0 100 5 10
    240613155110_g61h TGCAGGCTATCATACGAGGCTGTCATGCCCAG(+AGGACA)GATCCTTCCTAGCAGTCCCCATAGAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240613155108_5waf ATAGATCCTAAGATCCAGCAAGCTTGGCTCAG(+AGGACA)GATCCTGTTGGGGAGCAGCCACCTGCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240613155107_j7or CTGGGATAAGGCAGGTGGCTGCTCCCCAACAG(+AGGACA)GATCCTGAGCCAAGCTTGCTGGATCTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613155106_swir GAGCTGAAGCCCCTTAGGAGGTGCTGGCTCAG(+AGGACA)GTACCTCCCTGCGGGATAGGAAGGGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613155104_d0m5 CCTGGTCACTCCCCTTCCTATCCCGCAGGGAG(+AGGACA)GTACCTGAGCCAGCACCTCCTAAGGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613155103_ykty TTGTATAGGTTGTGTGCAGGGTCGGGAGGTTG(+AGGACA)GATCCCCTGGGTTGAGAGATGTGTTCTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613155100_zylh GTGGCTCACAGAACACATCTCTCAACCCAGGG(+AGGACA)GATCCAACCTCCCGACCCTGCACACAACCTA NGG 10 8 17 8 24 0 100 5 10
    240613155058_g3m3 TTCGGCACTAAGTGGCATCGGGGGGCGGGGAG(+AGGACA)GATCCCCAGGATGAGGACAGAGCCCAGCCCA NGG 10 8 17 8 24 0 100 5 10