Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155128_jnfj CCTGGTGAAAACAGTTGATGCTGAATATTGGG(+AGGACA)GATCCTGGTAGCCATGCTCCTGGAGCAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613155127_0ch9 CTGTAGCTGTCTTCAGAGACATTAGGTCAAAG(+AGGACA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613155126_gyz7 ACCACATGGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCTTTGACCTAATGTCTCTGAAGACAGC NGG 10 8 17 8 24 0 100 5 10
    240613155124_zjug AGCTCACAACCATTTGTAACTCCAATTTTAGG(+AGGACA)GATCCAGTGCCTAACTCTGGCTTCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613155123_p5rr CTGGTGCCTGTGGAAGCCAGAGTTAGGCACTG(+AGGACA)GATCCCTAAAATTGGAGTTACAAATGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613155121_gnp4 TGATACCAAGACCTAACAAACCCCGTGCCTGG(+AGGACA)GTACCTCTTTTAAAGCTCTGCACCCCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613155119_y34p CCTTGAAGAAGGGGTGCAGAGCTTTAAAAGAG(+AGGACA)GTACCCAGGCACGGGGTTTGTTAGGTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613155118_17jq ACAGCCCATCTGCCACAAGAGCTTCCTGATTG(+AGGACA)GTACCACTGTGAACCGTCCCTCCCCCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613155117_ogtb CTGTCTGGAGGGGGGAGGGACGGTTCACAGTG(+AGGACA)GTACCAATCAGGAAGCTCTTGTGGCAGATGG NGG 10 8 17 8 24 0 100 5 10
    240613155116_cdmp GTGCTTCAGGGTGGGCCCTATTTGGGCTTGAG(+AGGACA)GATCCCGTGGGGGCGGGGCTTCACCCCGACT NGG 10 8 17 8 24 0 100 5 10