Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155159_hbf5 GAGTTTTTTATTACAAAATTATTATTTTTATG(+AGGACA)GTACCTTTAACAAGGACCCTATGGCAAATGC NGG 10 8 17 8 24 0 100 5 10
    240613155157_ltpg TCTGCCTGCCTGCAGGTTGGCCATGCTCCTTG(+AGGACA)GATCCTGTAGGTTAACATTCATGCTTTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613155156_trg4 CTTCATCAGAAAGCATGAATGTTAACCTACAG(+AGGACA)GATCCAAGGAGCATGGCCAACCTGCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613155154_o8xr GACCTCTGGATCCTGTGATACTCTGTTGGCCG(+AGGACA)GTACCTACTGGGCTCCCTCAGCCCTATACCT NGG 10 8 17 8 24 0 100 5 10
    240613155152_p40k CCCACAGGTATAGGGCTGAGGGAGCCCAGTAG(+AGGACA)GTACCGGCCAACAGAGTATCACAGGATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613155150_oi94 TGATGTGGGCCTCCTTGGCTGTTGGACCTCTG(+AGGACA)GATCCTGTGATACTCTGTTGGCCGGTACCTA NGG 10 8 17 8 24 0 100 5 10
    240613155149_bi9d CCCAGTAGGTACCGGCCAACAGAGTATCACAG(+AGGACA)GATCCAGAGGTCCAACAGCCAAGGAGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613155148_35a4 GTATGTAAGCTGGACAAGATTCTACAGTAGAG(+AGGACA)GTACCTACATGCCTGCCAGGGGAACCGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613155147_hcpb AGATGACAACGGTTCCCCTGGCAGGCATGTAG(+AGGACA)GTACCTCTACTGTAGAATCTTGTCCAGCTTA NGG 10 8 17 8 24 0 100 5 10
    240613155145_907l TCAGACGTTGTTTTGACCAAAGTGTTCACTGG(+AGGACA)GATCCTCCAGGAAGTACCAGGGAACTGCAGC NGG 10 8 17 8 24 0 100 5 10