Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155144_ljsu GTTATGCTGCAGTTCCCTGGTACTTCCTGGAG(+AGGACA)GATCCCAGTGAACACTTTGGTCAAAACAACG NGG 10 8 17 8 24 0 100 5 10
    240613155142_uw90 CACATTAGTTAGTAACCACAGAACTGGTTTTG(+AGGACA)GATCCTGGAATTAAAGCATTAATATCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613155141_u5l6 ATTTACCTTTGATATTAATGCTTTAATTCCAG(+AGGACA)GATCCAAAACCAGTTCTGTGGTTACTAACTA NGG 10 8 17 8 24 0 100 5 10
    240613155139_dkdx GTGTCAGTCTCCAAGCTTACCTTTTATACAAG(+AGGACA)GATCCCAGTCTTGGGTTGGGGCTCACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613155138_7ib0 AGTAGACAGGGTGAGCCCCAACCCAAGACTGG(+AGGACA)GATCCTTGTATAAAAGGTAAGCTTGGAGACT NGG 10 8 17 8 24 0 100 5 10
    240613155136_bge6 GCTATTTCTTGCTCCAGGAGCATGGCTACCAG(+AGGACA)GATCCCCAATATTCAGCATCAACTGTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613155134_4q80 CCTGGTGAAAACAGTTGATGCTGAATATTGGG(+AGGACA)GATCCTGGTAGCCATGCTCCTGGAGCAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613155133_j8v0 CTGATACCTTCTTCAGCTCTTATTTAAGGAAG(+AGGACA)GTACCGTTTATGTTTCAAAACTTAGATATTC NGG 10 8 17 8 24 0 100 5 10
    240613155132_o7bl GCCCAGAATATCTAAGTTTTGAAACATAAACG(+AGGACA)GTACCTTCCTTAAATAAGAGCTGAAGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613155130_l1fl GCTATTTCTTGCTCCAGGAGCATGGCTACCAG(+AGGACA)GATCCCCAATATTCAGCATCAACTGTTTTCA NGG 10 8 17 8 24 0 100 5 10