Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155336_n6mj CAGGGAGGCTAATCAGATATATTGTACTTAGG(+AGGACA)GATCCTCAGTGCAGCTCATGCAGGACTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613155335_jnnv CACCCAACTAAGGGGCATGGATGCCCAGTGTG(+AGGACA)GATCCTGTGGGGATTCTGTTGTCTCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613155333_zuma ATGAAGGAAAGGAGACAACAGAATCCCCACAG(+AGGACA)GATCCACACTGGGCATCCATGCCCCTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613155331_09zq TGCAGGGCAGTGGGAGATGGTCTCATTTGGGG(+AGGACA)GTACCCTTTAGTGAACCTGTCTTCCCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240613155330_qw9g ATACTCTAAGGGGAAGACAGGTTCACTAAAGG(+AGGACA)GTACCCCCAAATGAGACCATCTCCCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240613155327_c2a9 GAGCATCCCGCCTTGCTCTCCTCCCCTCCCTG(+AGGACA)GATCCCCCCATTAAAGCTGGAGGTGGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613155325_49i7 CAAAGGCAGCCCACCTCCAGCTTTAATGGGGG(+AGGACA)GATCCAGGGAGGGGAGGAGAGCAAGGCGGGA NGG 10 8 17 8 24 0 100 5 10
    240613155324_efpk GTCTGCAGCTCCATTACTTTTCTCAGTCAGTG(+AGGACA)GATCCAAGGGACCAAGATCCATTGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240613155321_h5zi ATTTGCATCTGCCAATGGATCTTGGTCCCTTG(+AGGACA)GATCCACTGACTGAGAAAAGTAATGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613155319_k6pc AAAAAGCAGCGGCAGACAGGATTTTTGTCCAG(+AGGACA)GATCCACTATCCAGCTTTAAGATGAGTCATT NGG 10 8 17 8 24 0 100 5 10