Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205330_bygc GACATTTTTACCATCTGCTCTCTTCAGTATCG(+ACTCAC)GATCCGTATTGAGAAAAGAGAAGCACCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604205328_7pgc GCAGCGTCTGGTGCTTCTCTTTTCTCAATACG(+ACTCAC)GATCCGATACTGAAGAGAGCAGATGGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240604205327_jk2b GGGTCTGAGTTTGCTTTCCGTTGCTGTGGTGG(+ACTCAC)GTACCACGACTGGAGCAACATGGGGGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240604205325_kz1x TAAAGCCTTTCCCCCATGTTGCTCCAGTCGTG(+ACTCAC)GTACCCACCACAGCAACGGAAAGCAAACTCA NGG 10 8 17 8 24 0 100 5 10
    240604205323_rktm CTGTTGTGTCTAGCTTGTAGGTGTGCTCTGGG(+ACTCAC)GATCCCAAGTCAGGTCATCAGACTTGCACTG NGG 10 8 17 8 24 0 100 5 10
    240604205321_14o3 GCTTGCAGTGCAAGTCTGATGACCTGACTTGG(+ACTCAC)GATCCCCAGAGCACACCTACAAGCTAGACAC NGG 10 8 17 8 24 0 100 5 10
    240604205319_94zn CTGGAGTTATAGATGGTTGTGAGTTACCTGTG(+ACTCAC)GATCCTGAGAACTGAACCCAAGACTACAGGA NGG 10 8 17 8 24 0 100 5 10
    240604205317_xaqk GCTATTCCTGTAGTCTTGGGTTCAGTTCTCAG(+ACTCAC)GATCCACAGGTAACTCACAACCATCTATAAC NGG 10 8 17 8 24 0 100 5 10
    240604205316_1isx TTATTCTGTAGTTGGAGCCCAAACTCATTAAG(+ACTCAC)GTACCCTTTATGAGGCCAAGAGAGCCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604205314_0u0c ATGCCCCTCTGGCTCTCTTGGCCTCATAAAGG(+ACTCAC)GTACCTTAATGAGTTTGGGCTCCAACTACAG NGG 10 8 17 8 24 0 100 5 10