Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205348_sbsg AAGAATGTGTGTCATGGTAGAGGGGTTCCCAG(+ACTCAC)GATCCTCTCCTGCCAGATGCTTGGCTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240604205346_dahm GACCTGCAGCAGCCAAGCATCTGGCAGGAGAG(+ACTCAC)GATCCTGGGAACCCCTCTACCATGACACACA NGG 10 8 17 8 24 0 100 5 10
    240604205343_6kw9 CACCTACCCCGATGCCCGAGTTCAACTCCCAG(+ACTCAC)GATCCACTTGGTGGAAGGAGACCACTGATCC NGG 10 8 17 8 24 0 100 5 10
    240604205342_w33v TGTGGGGATCAGTGGTCTCCTTCCACCAAGTG(+ACTCAC)GATCCTGGGAGTTGAACTCGGGCATCGGGGT NGG 10 8 17 8 24 0 100 5 10
    240604205340_vl3y CCAGTTGGTACACTGTTTTCTGAAAGAGGTGG(+ACTCAC)GATCCAAAAGAGCTGTTTCTAGCTGCACTCC NGG 10 8 17 8 24 0 100 5 10
    240604205339_tkba TGCTTGGAGTGCAGCTAGAAACAGCTCTTTTG(+ACTCAC)GATCCCACCTCTTTCAGAAAACAGTGTACCA NGG 10 8 17 8 24 0 100 5 10
    240604205337_il9o TGCCAGTCTGTGTCTGTCTAATCAGAAGGATG(+ACTCAC)GATCCCTGTACCTAACATTCCATATCACTAC NGG 10 8 17 8 24 0 100 5 10
    240604205336_sbvp TCAGTGTAGTGATATGGAATGTTAGGTACAGG(+ACTCAC)GATCCATCCTTCTGATTAGACAGACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240604205334_nifl AAGGGCTATTCAAGCTCCCAAGAAAATCTGTG(+ACTCAC)GATCCATGGGTTACAGATTAAAGTTCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240604205331_a05m ACCCAGTTTGGAACTTTAATCTGTAACCCATG(+ACTCAC)GATCCACAGATTTTCTTGGGAGCTTGAATAG NGG 10 8 17 8 24 0 100 5 10