Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205459_eeu4 ATGTAGTGCCTTTTTTGTGTTCTGAGGGAGTG(+ACTCAC)GTACCCTGAGGTCTCGTCGTGTAGTTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240604205457_uyj8 TTAACTCTAAAACTACACGACGAGACCTCAGG(+ACTCAC)GTACCACTCCCTCAGAACACAAAAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240604205455_ea4m TGCCAAGAGCTGGTAAGCGACACAACCCATGG(+ACTCAC)GTACCAGGGATTCTGGCTGACACAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240604205452_32b4 TCAGGTTCACCTTGTGTCAGCCAGAATCCCTG(+ACTCAC)GTACCCATGGGTTGTGTCGCTTACCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604205451_lgh2 TACTCACGGTCTCTGTTTTCTCACTCTCTGAG(+ACTCAC)GTACCACAGGCAGGCAGCCCTTGAGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240604205449_f0al CATTGGCTGAGCTCAAGGGCTGCCTGCCTGTG(+ACTCAC)GTACCTCAGAGAGTGAGAAAACAGAGACCGT NGG 10 8 17 8 24 0 100 5 10
    240604205447_i4gc AATAGTGAGCGGGGAAAGGAGAATAGGAAGTG(+ACTCAC)GATCCTTCCAGAACCTACCTCTCTCTGAAGC NGG 10 8 17 8 24 0 100 5 10
    240604205445_4m0p GTTGAGCTTCAGAGAGAGGTAGGTTCTGGAAG(+ACTCAC)GATCCACTTCCTATTCTCCTTTCCCCGCTCA NGG 10 8 17 8 24 0 100 5 10
    240604205444_soly CCTCTCTTTACGTGAGATACTGTGGCGTGCTG(+ACTCAC)GTACCATCCAAGCCTCGGCCCGGTGTCTATC NGG 10 8 17 8 24 0 100 5 10
    240604205442_5viq GAGGGGATAGACACCGGGCCGAGGCTTGGATG(+ACTCAC)GTACCAGCACGCCACAGTATCTCACGTAAAG NGG 10 8 17 8 24 0 100 5 10