Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205534_c4o8 GTCAACTCTCCAGGACAACACTAACCTCTTGG(+ACTCAC)GATCCTATGGAGAGAAAAAGGAAGTGTGACA NGG 10 8 17 8 24 0 100 5 10
    240604205532_0jvf ACAGTTGTCACACTTCCTTTTTCTCTCCATAG(+ACTCAC)GATCCCAAGAGGTTAGTGTTGTCCTGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240604205530_cdbi TTCTCCTGAGTTTCCCCTGGGTGTAACTCCTG(+ACTCAC)GATCCCCTGAGGATCTTCCCACACTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604205528_ovdc GCTAAACCTCCAGTGTGGGAAGATCCTCAGGG(+ACTCAC)GATCCAGGAGTTACACCCAGGGGAAACTCAG NGG 10 8 17 8 24 0 100 5 10
    240604205526_msqt GCAAGTCAGCTCTGGATCCCAAAGGGGGCCTG(+ACTCAC)GTACCACCCACACCGCTTCTTAGAAGGATCC NGG 10 8 17 8 24 0 100 5 10
    240604205524_8ucy TTTCTGGATCCTTCTAAGAAGCGGTGTGGGTG(+ACTCAC)GTACCAGGCCCCCTTTGGGATCCAGAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604205523_w6gi CAGAACCCAGACTCTTACCCTTACCCAAAATG(+ACTCAC)GATCCAGTTCCACTGCCTCCCTCTCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240604205522_acnp CAGTGAGGAGGGAGAGGGAGGCAGTGGAACTG(+ACTCAC)GATCCATTTTGGGTAAGGGTAAGAGTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604205520_xcjb TATCCTTTCTGTTTTGGGCTCTACAGCCTCTG(+ACTCAC)GATCCACTGGGCCTTGGCAACCCTTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240604205518_dr4w GAGCCAAGAACAAGGGTTGCCAAGGCCCAGTG(+ACTCAC)GATCCAGAGGCTGTAGAGCCCAAAACAGAAA NGG 10 8 17 8 24 0 100 5 10