Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205628_87w8 TGTCTTAGTGCAGCAGAAGATAGTTCCAGCAG(+ACTCAC)GTACCGGCTGGGCTTGGTGGCTTTCCAAGCG NGG 10 8 17 8 24 0 100 5 10
    240604205627_45j7 ACGTGCGCTTGGAAAGCCACCAAGCCCAGCCG(+ACTCAC)GTACCTGCTGGAACTATCTTCTGCTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240604205625_2ixp CCATGAATATTAGTGTCCAACAGGTGGAGTCG(+ACTCAC)GTACCCACATTCCTACCCCGCCCCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240604205623_3vhq AGTGCCAGGTCTGGGGCGGGGTAGGAATGTGG(+ACTCAC)GTACCGACTCCACCTGTTGGACACTAATATT NGG 10 8 17 8 24 0 100 5 10
    240604205621_eazo GGGTGCAGGCAGCTGTAGAATCCCAAAGAAGG(+ACTCAC)GATCCAAACAATGTTTGAAGACCCCACCCCA NGG 10 8 17 8 24 0 100 5 10
    240604205620_0uv7 TAAATTGGGGTGGGGTCTTCAAACATTGTTTG(+ACTCAC)GATCCCTTCTTTGGGATTCTACAGCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604205618_nj9x CAAGTAAATGGCCTTGAAGTGGCCATGCCTTG(+ACTCAC)GTACCGAGCAGTCAAACTGATGAACACGGCA NGG 10 8 17 8 24 0 100 5 10
    240604205617_ipju ACAAATGCCGTGTTCATCAGTTTGACTGCTCG(+ACTCAC)GTACCAAGGCATGGCCACTTCAAGGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240604205615_vmye CTATCTAGATGGACCTGTATAATGTCTGCCTG(+ACTCAC)GTACCTGAGATCTAGCCTCTGCACTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604205614_iosh ATTTGCCACTAAGTGCAGAGGCTAGATCTCAG(+ACTCAC)GTACCAGGCAGACATTATACAGGTCCATCTA NGG 10 8 17 8 24 0 100 5 10