Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205551_fdma GCTGTGAGGAAGGTCCTGTGCCGACACAGTGG(+ACTCAC)GTACCTGGCAAGGGTAAGAGGAGAGCCATCA NGG 10 8 17 8 24 0 100 5 10
    240604205550_hddj GCTGGTGATGGCTCTCCTCTTACCCTTGCCAG(+ACTCAC)GTACCCACTGTGTCGGCACAGGACCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240604205548_cucr GGGTCTCTGGGTGATGAGGTCAGAGTCTCTGG(+ACTCAC)GATCCCCTGAAATCCCGGAGAAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604205546_y83s TTCTTCCCAGGCTCTTCTCCGGGATTTCAGGG(+ACTCAC)GATCCCAGAGACTCTGACCTCATCACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604205545_cwyz TCACAACCATCTTTGTAACTCCAGTTTCAAGG(+ACTCAC)GATCCAACCCCCTCTCCTGACCTCAAACACC NGG 10 8 17 8 24 0 100 5 10
    240604205543_a12y AGTCTGGTGTTTGAGGTCAGGAGAGGGGGTTG(+ACTCAC)GATCCCTTGAAACTGGAGTTACAAAGATGGT NGG 10 8 17 8 24 0 100 5 10
    240604205541_8oxw GCATTGGAGTTTCAAATCCCGCAGTCCTGCGG(+ACTCAC)GTACCACATTCCTAATGGAGCCTTTTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240604205540_h7w1 ATCACAGGCCAAAAGGCTCCATTAGGAATGTG(+ACTCAC)GTACCCGCAGGACTGCGGGATTTGAAACTCC NGG 10 8 17 8 24 0 100 5 10
    240604205538_t6ls CCCCCTCCCCCAAGCCTGGGCATGGAATGTGG(+ACTCAC)GATCCCCAGGCCCTGCCCAGAGCTGGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240604205535_7dkm GGACCCCTCCCCAGCTCTGGGCAGGGCCTGGG(+ACTCAC)GATCCCACATTCCATGCCCAGGCTTGGGGGA NGG 10 8 17 8 24 0 100 5 10