Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205700_m6hy GGATGTCCTTCCAGGTCAATAATCTTAGCAGG(+ACTCAC)GTACCTTCCTCAGAATGGCCAGAGCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240604205659_epol CTCCTTCTCCAGCTCTGGCCATTCTGAGGAAG(+ACTCAC)GTACCCTGCTAAGATTATTGACCTGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604205656_l959 CACCAGAAAGAGATCATGATGTGCCAGCCAGG(+ACTCAC)GATCCTGCGACCCAGATCTTCTCTCCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240604205655_3c92 AACTGTCTCTGGAGAGAAGATCTGGGTCGCAG(+ACTCAC)GATCCCTGGCTGGCACATCATGATCTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240604205654_epst GGGGGGGGGGGCTGGGTTTTTGGAATCTGTAG(+ACTCAC)GATCCATAGCACAATCATCTACCACACACCT NGG 10 8 17 8 24 0 100 5 10
    240604205653_yr02 GAAGTAGGTGTGTGGTAGATGATTGTGCTATG(+ACTCAC)GATCCTACAGATTCCAAAAACCCAGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240604205651_3cdq CTCCTCTGGGGAGCCTTTCCATAGCAGCTCAG(+ACTCAC)GTACCAAAGAGGCAAGGAAAGAGCAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240604205650_g8wd CCAGGAACCCCTGCTCTTTCCTTGCCTCTTTG(+ACTCAC)GTACCTGAGCTGCTATGGAAAGGCTCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604205648_ypun GGGAGCCAGCAGAGACCAAAGGGAAGGAGGAG(+ACTCAC)GTACCTGAGGCTTGGCAGGATCCCCAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240604205645_xwfu CAAGGACCCCTGGGGATCCTGCCAAGCCTCAG(+ACTCAC)GTACCTCCTCCTTCCCTTTGGTCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10