Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205643_3rmc GGACCTGGATGTTTCAGGCAATCTGCTGCCTG(+ACTCAC)GTACCTATTACTGTCACTATCCCCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240604205641_5g0v GGCCACCATGCTGGGGATAGTGACAGTAATAG(+ACTCAC)GTACCAGGCAGCAGATTGCCTGAAACATCCA NGG 10 8 17 8 24 0 100 5 10
    240604205640_c0mj CATGTGTGCCTGGTGCCAGAGGTCAGAAAAGG(+ACTCAC)GTACCACCTCCCCTGGTACTGGAATTTAGGT NGG 10 8 17 8 24 0 100 5 10
    240604205638_pd2q GAACTACCTAAATTCCAGTACCAGGGGAGGTG(+ACTCAC)GTACCCTTTTCTGACCTCTGGCACCAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240604205637_ff4z TGGAAAAGCTTGGCAGGCCCCGGGAGGACTCG(+ACTCAC)GATCCATCCCTTTATCTCATCCCTACGCTCC NGG 10 8 17 8 24 0 100 5 10
    240604205636_pr4m ATACTGGAGCGTAGGGATGAGATAAAGGGATG(+ACTCAC)GATCCGAGTCCTCCCGGGGCCTGCCAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604205635_va5j ATCTGTCCCATTTCAGAGAGGGCCCTTACTAG(+ACTCAC)GTACCTTTGGTGTGGGGGGCGCTTGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240604205633_tczd AGCTTGTTCCCCAAGCGCCCCCCACACCAAAG(+ACTCAC)GTACCTAGTAAGGGCCCTCTCTGAAATGGGA NGG 10 8 17 8 24 0 100 5 10
    240604205631_hwtl GGATGTAAACACACTCTGTAAGCTGAAATTCG(+ACTCAC)GTACCCAAATGCATAAGTGGACTTATGACCA NGG 10 8 17 8 24 0 100 5 10
    240604205630_wbmq TGACATGGTCATAAGTCCACTTATGCATTTGG(+ACTCAC)GTACCGAATTTCAGCTTACAGAGTGTGTTTA NGG 10 8 17 8 24 0 100 5 10