Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205313_6mzl TGGGGGTTCAGGCTCCAGGATCAATTTAGAGG(+ACTCAC)GATCCTTGAACCCAGCCCCGTGAACAATCTA NGG 10 8 17 8 24 0 100 5 10
    240604205311_1myf TATGATAGATTGTTCACGGGGCTGGGTTCAAG(+ACTCAC)GATCCCTCTAAATTGATCCTGGAGCCTGAAC NGG 10 8 17 8 24 0 100 5 10
    240604205310_9hso GACTAACATGCCCAGGTGGTGTCGGCTCAGAG(+ACTCAC)GATCCTAGCACCATCTTAAGCAGGTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240604205309_lt08 CCCTGCCTCCCACCTGCTTAAGATGGTGCTAG(+ACTCAC)GATCCTCTGAGCCGACACCACCTGGGCATGT NGG 10 8 17 8 24 0 100 5 10
    240604205306_b25p TGTTCAGTAAACTCTGGCTGCTTTGAGGAGGG(+ACTCAC)GATCCTGCTGTCCCAGGGAACCAGGACATGT NGG 10 8 17 8 24 0 100 5 10
    240604205305_r8m1 CAAGGACATGTCCTGGTTCCCTGGGACAGCAG(+ACTCAC)GATCCCCTCCTCAAAGCAGCCAGAGTTTACT NGG 10 8 17 8 24 0 100 5 10
    240604205304_lt9m GCAGTCCCCACGCAGGGTGGCTTCCTGTCGAG(+ACTCAC)GATCCAGTGGGCCCAGCGTTGGTCCACCCAC NGG 10 8 17 8 24 0 100 5 10
    240604205301_1xgm ACTGAGTGGGTGGACCAACGCTGGGCCCACTG(+ACTCAC)GATCCTCGACAGGAAGCCACCCTGCGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604205259_ijou GGCAGGTATCAGAGGTGAAGGTATTCTGGTGG(+ACTCAC)GATCCCCCTCCCAAGGGACCTTGGCTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240604205257_hizt CTAGGACTCCAGCCAAGGTCCCTTGGGAGGGG(+ACTCAC)GATCCCACCAGAATACCTTCACCTCTGATAC NGG 10 8 17 8 24 0 100 5 10