Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200106_6915 ATCAGATTGACACCCAAAACATCCTGTTTTGG(+ACTCAC)GATCCTGAGGCTCCACGATCAAGGTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240604200105_ai1n TGGAAGAGTTGTGTCCTTCCCCTACGAAGGTG(+ACTCAC)GTACCGATGTGCTCTGCCTTTCTGATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240604200103_kdme ATAAAATGGGATCAGAAAGGCAGAGCACATCG(+ACTCAC)GTACCACCTTCGTAGGGGAAGGACACAACTC NGG 10 8 17 8 24 0 100 5 10
    240604200102_cyec CACACACGTGCATGCTCTGTGCGTGTATGTGG(+ACTCAC)GTACCGTGTATATGTGTGTGAGTGTTGTATA NGG 10 8 17 8 24 0 100 5 10
    240604200100_wt56 ATGCATATACAACACTCACACACATATACACG(+ACTCAC)GTACCCACATACACGCACAGAGCATGCACGT NGG 10 8 17 8 24 0 100 5 10
    240604200058_yek1 CTACTGATACTTTCAGGGGTGGTCCCATGGGG(+ACTCAC)GATCCATGCAGTGGAAGAAACACTGGATTGG NGG 10 8 17 8 24 0 100 5 10
    240604200057_lj7j CGTACCCAATCCAGTGTTTCTTCCACTGCATG(+ACTCAC)GATCCCCCATGGGACCACCCCTGAAAGTATC NGG 10 8 17 8 24 0 100 5 10
    240604200055_3ky7 ATGTAATTAGGCTTTTTGAGGTAGTTCTCAAG(+ACTCAC)GATCCTCTTTTGACTCTTGGGAAAGAATTGT NGG 10 8 17 8 24 0 100 5 10
    240604200054_l5h5 CACGCACAATTCTTTCCCAAGAGTCAAAAGAG(+ACTCAC)GATCCTTGAGAACTACCTCAAAAAGCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240604200053_zo74 ATGAGGAGTAATCTCCTGTTATTTTCTATTTG(+ACTCAC)GTACCAAGATAGCTAATATTACGTGAAATTT NGG 10 8 17 8 24 0 100 5 10