Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200051_r3a9 CCAGAAAATTTCACGTAATATTAGCTATCTTG(+ACTCAC)GTACCAAATAGAAAATAACAGGAGATTACTC NGG 10 8 17 8 24 0 100 5 10
    240604200050_eazo TTTTCTGGCCCTTTTAAGGGTGCCTACAGTAG(+ACTCAC)GATCCAAAGAGCAGCCCAGCACTCGATTTCT NGG 10 8 17 8 24 0 100 5 10
    240604200048_1lzi ATCCTAGAAATCGAGTGCTGGGCTGCTCTTTG(+ACTCAC)GATCCTACTGTAGGCACCCTTAAAAGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240604200047_ixs9 AAAGAGGAAAAAGCCTACAACCCTCTCTGCTG(+ACTCAC)GATCCATCCCCACCTCCTCCGGTCAGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240604200044_eovl TTCTGGAACCCTGACCGGAGGAGGTGGGGATG(+ACTCAC)GATCCAGCAGAGAGGGTTGTAGGCTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240604200043_r5tt CAGATGCCTCCAGCACTGCCATGTGCCCAGTG(+ACTCAC)GTACCCCTTGACAGTGTTGTTCTCAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240604200041_qwf4 GAACAAGTCACTGAGAACAACACTGTCAAGGG(+ACTCAC)GTACCACTGGGCACATGGCAGTGCTGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240604200039_jdcw ATAGACTCGGATCCTTCCTGCATTGTTGCAAG(+ACTCAC)GTACCCAATTGTCAGTTGCAGGAGACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604200038_ocfv CACACTCTGGGTCTCCTGCAACTGACAATTGG(+ACTCAC)GTACCTTGCAACAATGCAGGAAGGATCCGAG NGG 10 8 17 8 24 0 100 5 10
    240604200036_xfv8 TCTGCATAGTTTAAAACCACATGGATCATCTG(+ACTCAC)GTACCTTTTTTTCCTAGACAACTTTGATCCT NGG 10 8 17 8 24 0 100 5 10