Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195948_ecyg AGCCCCAAGAGACCCTGAAAAGGCTGTGCATG(+ACTCAC)GATCCCCCCTCTGCTTCCGTGGTGGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240604195946_aypa TCACATCTCCCTTCAGAAATCATGTTTGCAGG(+ACTCAC)GATCCGGTTTTGTATTTTGTTTTTAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240604195944_zr70 TTAGACTTGTTTAAAAACAAAATACAAAACCG(+ACTCAC)GATCCCTGCAAACATGATTTCTGAAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240604195943_t8ja GGACCTTTATTTACCTAACTTAAGATCATTAG(+ACTCAC)GTACCTGGTTTGGCTTACACTCAAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604195941_hbpz GCAGACCTGGGTTTGAGTGTAAGCCAAACCAG(+ACTCAC)GTACCTAATGATCTTAAGTTAGGTAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240604195940_5xzu GTTGGGGGTGGGGTGTTACATGCATGTCACTG(+ACTCAC)GATCCTATGGAAGTTGTGAGCTGCCATGTAG NGG 10 8 17 8 24 0 100 5 10
    240604195937_xmkn AGCAGCTACATGGCAGCTCACAACTTCCATAG(+ACTCAC)GATCCAGTGACATGCATGTAACACCCCACCC NGG 10 8 17 8 24 0 100 5 10
    240604195936_jmh2 AGAGGGCCAATGAAACCCAAAGGTGAGAAAGG(+ACTCAC)GATCCTGGGCACAAGTGAGATAAACTTAATC NGG 10 8 17 8 24 0 100 5 10
    240604195933_5481 CTTAGGATTAAGTTTATCTCACTTGTGCCCAG(+ACTCAC)GATCCCTTTCTCACCTTTGGGTTTCATTGGC NGG 10 8 17 8 24 0 100 5 10
    240604195932_y514 GCAGTTTTGTGGCAGAATGGGCCATTGTTGTG(+ACTCAC)GATCCATCCAAACGTATCCCCAGCTAAAATA NGG 10 8 17 8 24 0 100 5 10