Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195854_odf7 AATCAACTGCTCTGGTTTACTTCATATTTGTG(+ACTCAC)GATCCCCACCTCTTGAGTATTTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604195853_v41y AGTAAGAGTTCCATTTTCTCACATCAGTGAAG(+ACTCAC)GTACCATACATTGAAACTCTCAGTACTATAT NGG 10 8 17 8 24 0 100 5 10
    240604195851_e8q7 TAGAAATATAGTACTGAGAGTTTCAATGTATG(+ACTCAC)GTACCTTCACTGATGTGAGAAAATGGAACTC NGG 10 8 17 8 24 0 100 5 10
    240604195850_p3bk AGAATTCAATATAGTTGTTGCTGTGAATATAG(+ACTCAC)GTACCATTTTGGGTCATCTACACTAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240604195849_2fyn TGTGTCAGTCTTAGTGTAGATGACCCAAAATG(+ACTCAC)GTACCTATATTCACAGCAACAACTATATTGA NGG 10 8 17 8 24 0 100 5 10
    240604195847_0mpo TAGTTTATCACATTGAAATAAGAATCTCTTTG(+ACTCAC)GTACCTTTTATTCAGTAAATCTTAACAATGA NGG 10 8 17 8 24 0 100 5 10
    240604195846_nyxj TTCTGTCATTGTTAAGATTTACTGAATAAAAG(+ACTCAC)GTACCAAAGAGATTCTTATTTCAATGTGATA NGG 10 8 17 8 24 0 100 5 10
    240604195845_nclg CAAGTGGAATGACTAAATGACCCAGCTGTTTG(+ACTCAC)GATCCAGGCAGCTTGGGTATCCATGGGGTTA NGG 10 8 17 8 24 0 100 5 10
    240604195843_pe98 CAGGCTAACCCCATGGATACCCAAGCTGCCTG(+ACTCAC)GATCCAAACAGCTGGGTCATTTAGTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240604195841_nebl AACTTTTGGGTTTGAGCTACAAACTACACCAG(+ACTCAC)GTACCACCCCAGATAGAGGGTGTCTTGGGTG NGG 10 8 17 8 24 0 100 5 10