Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200251_ww2x TGTCCACACATCCCTCTCTAAGCCCAGGATAG(+ACTCAC)GTACCAATATAGGTAACTCTGCATTTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604200250_ctf6 CTTATGGGAAAAATGCAGAGTTACCTATATTG(+ACTCAC)GTACCTATCCTGGGCTTAGAGAGGGATGTGT NGG 10 8 17 8 24 0 100 5 10
    240604200248_dorb TACACCTCTAGGAACTGAGGCAGCTTCAGAAG(+ACTCAC)GTACCTGCCAGATGTTGCTGTGGGAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240604200247_lre4 AAGGCCATGCTTCCCACAGCAACATCTGGCAG(+ACTCAC)GTACCTTCTGAAGCTGCCTCAGTTCCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240604200244_ps7h GATGGCTTACAACTGTAACCCTGGTTCCAGGG(+ACTCAC)GATCCAGTACCTTCTGACCTGCTCGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604200243_j040 TTCCTGGCACCCGAGCAGGTCAGAAGGTACTG(+ACTCAC)GATCCCCTGGAACCAGGGTTACAGTTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240604200241_mwwa TTCCTTGAAGTGAGGTCAAGGTCATGTGGCCG(+ACTCAC)GATCCTATACTCACCACTGGCTGTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240604200240_vz3k CTTGAGAGAAAGACAGCCAGTGGTGAGTATAG(+ACTCAC)GATCCGGCCACATGACCTTGACCTCACTTCA NGG 10 8 17 8 24 0 100 5 10
    240604200239_58jc TTATCCACAGGACCTGGGTGGAGCAGGATATG(+ACTCAC)GATCCCTCATACTGAAAGATGTAGGGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604200237_w7f9 ACTTCCTTTTCCCTACATCTTTCAGTATGAGG(+ACTCAC)GATCCATATCCTGCTCCACCCAGGTCCTGTG NGG 10 8 17 8 24 0 100 5 10