Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195839_56l6 GCAGTCACCCAAGACACCCTCTATCTGGGGTG(+ACTCAC)GTACCTGGTGTAGTTTGTAGCTCAAACCCAA NGG 10 8 17 8 24 0 100 5 10
    240604195837_k2g1 CTGTGTGTATGTGCATGCAAACTCAGAGGAAG(+ACTCAC)GTACCAGGCTCCCTGGGACTAGAGTTATGGA NGG 10 8 17 8 24 0 100 5 10
    240604195835_2f1e CGCCATCCATAACTCTAGTCCCAGGGAGCCTG(+ACTCAC)GTACCTTCCTCTGAGTTTGCATGCACATACA NGG 10 8 17 8 24 0 100 5 10
    240604195830_qx86 TGACCCAGCGCTGTGCAAGGGTTGGTTCTGGG(+ACTCAC)GATCCAAGAACGTGTATTTGCTGTTATCTCT NGG 10 8 17 8 24 0 100 5 10
    240604195825_u1uj CGCCCAGAGATAACAGCAAATACACGTTCTTG(+ACTCAC)GATCCCCAGAACCAACCCTTGCACAGCGCTG NGG 10 8 17 8 24 0 100 5 10
    240604195824_lb7s ATTCAGTAACTTAAATATTGCCAGTGACTGAG(+ACTCAC)GATCCCCTCCAGTCATGGTTCTGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240604195822_unnc CTCAAAGAATATACAGAACCATGACTGGAGGG(+ACTCAC)GATCCTCAGTCACTGGCAATATTTAAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240604195821_jn24 ATAGATACTTTGCATTTATGGCTTGAATGAAG(+ACTCAC)GTACCAATTTTGATCTGGAGTTCTAAATGTG NGG 10 8 17 8 24 0 100 5 10
    240604195819_f6cu ATTTTCACATTTAGAACTCCAGATCAAAATTG(+ACTCAC)GTACCTTCATTCAAGCCATAAATGCAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240604195818_chxq GGCGGCTGTGGTCCAGCAGGATCTACATGTAG(+ACTCAC)GTACCTACTCTTTGGTGCAATAGTGTTACGA NGG 10 8 17 8 24 0 100 5 10